Biological Control of the Black Weevil (Cosmopolites sordidus) with the Entomopathogenic Nematode Heterorhabditis bacteriophora Under Greenhouse Conditions in Banana Cultivation

Authors

  • Reina Medina Litardo University of Guayaquil, Agricultural Technology Institute of Vinces
  • Galo Salcedo Rosales University of Guayaquil, Agricultural Technology Institute of Vinces
  • Erika Tapia Rosado University of Guayaquil, Agricultural Technology Institute of Vinces

DOI:

https://doi.org/10.53591/iti.v2i2.1155

Keywords:

biological control, nematodes, Heterorhabditis bacteriophora, Cosmopolites sordidus, mortality, efficiency

Abstract

The objective of this study was to evaluate the effect of the nematode Heterorhabditis bacteriophora on larvae of the black weevil (Cosmopolites sordidus) under greenhouse conditions. A bioassay was conducted, involving direct inoculation of the nematode into the rhizome area of banana plants infested with black weevil larvae. The tested doses were 0, 75,000, 100,000, and 125,000 nematodes/m², arranged in a randomized complete block design. The number of dead larvae per plant was recorded at six and ten days after nematode application. The evaluated variables included larval mortality, nematode multiplication, and dose efficiency. The dose that caused the highest mortality in black weevil larvae was 125,000 nematodes/m², resulting in 91.67% dead larvae ten days after application. When analyzing dose efficiency, all concentrations were effective at ten days, with 125,000 nematodes/m² showing the highest efficacy (93.33% dead larvae), compared to the control group, which exhibited no larval mortality.

References

Barrio s, L. (2004). Evaluación del parasitismo de Heterorhabditis spp y Diplogasteritus spp sobre Phyllophaga spp, Scaptocoris Talpa, y Ampedus spp. a nivel de laboratorio e invernadero. Tesis de Doctorado. Guatemala.

Bleiholder, H. 1996. Métodos de Planteamiento y Valoración de ensayos de campo con Pes tici das. 2 ed. p.170.

Femandez L. (1994). El banano en el Ecuador. Cultivo, plagas y enfermedades. Guayaqu il, EC. p. 239.

Gaugler, R. (1999). Ecologic al considerations in the biological control of soil inhabiting insec ts with entomopatogenic nematodes. Agric. Ecosys. Environ. 24, 351-60.

Gold, C.S. & Messiaen, S. (2000). El picudo negro del banano Cosmopolites sordidus. Plagas de musa. Hoja divulgativa Nº 4. Solicitado 23 de diciembre, 2006, disponible e n http:// www.inibap.com.

Gonzales, G. 1976. Métodos estadísticos y principios de diseño experimental. Universidad Central. Ecuador. Quito. p 222.

lnstitut National de la Recherche Agronomique Antilles Guyane. (INRA). (1996). Los nematodos en el control biológico. Mimeografiado. 23 p.

Instituto Nacional de Meteorolog ía e Hidrología. Anuario Meteorológico. Ecuador, 2008. p 95.

Kaya, H. & Stock, S. 1997. Techniques in insect nematology, pp. 281- 3 24. En: Lacey, L. A. (ed.). Manual of techniques in insect pathology. Biological techniques. Academic Press, lnc. San Diego. Chap. 6.

Sepúlveda, P & Soto, J & López, B. 2004. Evaluación de la virulencia de Steinemema carpocapsae Ali Strain y Heterorhabditis bacteriophora, sobre los estados de desarrollo del picudo negro del plátano (Cosmopo li tes sordidus) Germar.

SICA. Producción del banano. Solicitado 20 de octubre, 2006, Disponible en http://www.sica.gov.ec.

Published

2010-12-30

Issue

Section

Artículos

Similar Articles

1 2 3 > >> 

You may also start an advanced similarity search for this article.