The Halotolerance and enzymatic synthesis by Pseudomonas cf. fluorescens isolated from Ecuasal salt ponds, province of Santa Elena, Ecuador

Abstract

The goal of this research is to determine the halotolerance and enzymatic synthesis of Pseudomonas cf. fluorescens. To evaluate halotolerance, cell growth was quantified in 2 M, 3 M and 4 M NaCl at 30°C. Also, the extracellular proteolytic enzyme synthesis, amylolytic and lipolytic was evaluated by halos of production. The treatment of 4 M to 30°C was the best Halotolerance assay, with a growth rate (μ) 0.048 h-1, doubling time (td) 14.55 h and a cell density of 5 906 666 ± 305 505 cell/mL. Proteolytic synthesis was the highest (halo 30 ± 0.82 mm), followed by the production of amylases (halo of 22.5±1.73 mm), the lipolytic activity was not observed.